The study area included the cities of Basel, Lugano and Zurich. The sampling design reflected four main city districts related to four categories of tree cover (Figure 1): 1) industrial/commercial areas, 0-20% tree cover; 2) residential areas, 20-40% tree cover; 3) urban parks and cemeteries, 40-60% tree cover; 4) peri-urban forests (natural/semi-natural forests), 60-100% tree cover. The tree cover gradient was evaluated across a square grid with a square side length of 100 m. For each square, we evaluated the area covered by urban trees using the Urban Atlas Street Tree Layer 2018 (European Union’s Copernicus Land Monitoring Service information, 2021).
In each city, we established eight transects of 1.2 km, two for each tree cover category. Transects crossed contiguous grid squares belonging to the same tree cover categories as much as possible. We performed aural-visual point counts of birds every 200 m along each transect (six points per transect) during the breeding season (April-June 2024) (Bibby et al., 2000). The transects were walked at the time of highest activity of birds, which is from thirty minutes after sunrise until 4 hours later. Each transect was walked twice, 20-60 days apart, to account for imperfect detection of birds and sample completeness (Balestrieri et al., 2017; Kéry et al., 2005).
